d7f10 xp  (Cell Signaling Technology Inc)

Journal: Stem Cell Research & Therapy

Article Title: Vagal α7nAChR signaling regulates α7nAChR + Sca1 + cells during lung injury repair

doi: 10.1186/s13287-020-01892-4

Figure Lengend Snippet: Effects of vagotomy or α7nAChR knockout on p-Akt1 + VE-cadherin + cells in pneumonia at 2 dpi. a Immunofluorescent images of α7nAChR and p-Akt1 expression in the bone marrow cells. b Flow cytometry analysis of p-Akt1 + cells in the bone marrow from wild-type and α7nAChR knockout mice with pneumonia at 2 dpi. c Immunofluorescence analysis of the coexpression of p-Akt1 and VE-cadherin in the bone marrow from sham and vagotomized pneumonia mice at 2 dpi. d Immunofluorescence analysis of the coexpression of p-Akt1 and VE-cadherin in the bone marrow from saline- and nicotine-treated pneumonia mice at 2 dpi

Article Snippet: Phospho-Akt1 (Ser473) (D7F10) XP® rabbit mAb (Akt1 Specific) and PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Knock-Out, Expressing, Flow Cytometry, Immunofluorescence, Saline

Journal: Stem Cell Research & Therapy

Article Title: Vagal α7nAChR signaling regulates α7nAChR + Sca1 + cells during lung injury repair

doi: 10.1186/s13287-020-01892-4

Figure Lengend Snippet: Knockout of Akt1 affects Sca1 + cells in the bone marrow and BAL in pneumonia. a Effect of Akt1 knockout on Sca1 + cells in the bone marrow from pneumonia mice at 2, 3, and 4 dpi. BM cells were isolated from wild-type and Akt1 knockout pneumonia mice at 2, 3, and 4 dpi for flow cytometry analysis. Normal cells were used as controls. b Effect of Akt1 knockout on Sca1 + cells in BAL from pneumonia mice at 2, 3, and 4 dpi. BAL cells were isolated from wild-type and Akt1 knockout pneumonia mice at 2, 3, and 4 dpi for flow cytometry. Normal cells were used as controls

Article Snippet: Phospho-Akt1 (Ser473) (D7F10) XP® rabbit mAb (Akt1 Specific) and PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Knock-Out, Isolation, Flow Cytometry

Journal: Stem Cell Research & Therapy

Article Title: Vagal α7nAChR signaling regulates α7nAChR + Sca1 + cells during lung injury repair

doi: 10.1186/s13287-020-01892-4

Figure Lengend Snippet: Flk1 expression in the α7nAChR + Sca1 + VE-cadherin + cell population and the effect of vagotomy on α7nAChR + Sca1 + VE-cadherin + p-Akt1 + cells in BM and lungs from sham and vagotomized mice. a Flow cytometric analysis of α7nAChR + Sca1 + VE-cadherin + Flk1 + cells in normal bone marrow cells. b The cell percentage analyzed from Fig. 7a. c Flow cytometric analysis of α7nAChR + Sca1 + VE-cadherin + Flk1 + cells in normal lung cells. d The cell percentage analyzed from Fig. 7c. e , f Effect of vagotomy on α7nAChR + Sca1 + VE-cadherin + p-Akt1 + cells in BM cells. N = 3 in each group. Student’s t test, * P < 0.05. g , h Effect of vagotomy on α7nAChR + Sca1 + VE-cadherin + p-Akt1 + cells in lung cells. N = 6 in each group. Student’s t test, * P < 0.05

Article Snippet: Phospho-Akt1 (Ser473) (D7F10) XP® rabbit mAb (Akt1 Specific) and PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Expressing